Heck J D, Costa M
Cancer Lett. 1982 Jan;15(1):19-26. doi: 10.1016/0304-3835(82)90071-4.
The incidence of morphological transformation following exposure of Syrian hamster embryo (SHE) cells to crystalline alphaNiS particles was considerably greater than that following a similar exposure to amorphous NiS particles. These differences in potency were attributable to the selective phagocytosis of crystalline alphaNiS particles into cells, since untreated amorphous NiS particles were not readily taken up. Chemical reduction of the amorphous NiS particles' surface with LiAlH4 resulted in an increase both in their phagocytic uptake by Chinese hamster ovary (CHO) cells and in their ability to induce transformation in SHE cells. The phagocytosis and transforming activity of crystalline alphaNiS particles was also enhanced by LiAlH4 reduction. These results are consistent with previous observations showing that untreated crystalline NiS particles have a negative surface charge while amorphous NiS particles possess positively charged surfaces. These findings support the general hypothesis that the transforming activity of particulate metal compounds is proportional to their phagocytic uptake. Specifically, these observations show that the entry of metal sulfide particles into cells is related to their surface properties and, in particular, to the degree of negative charge on the surface microenvironment.
将叙利亚仓鼠胚胎（SHE）细胞暴露于结晶态α-NiS颗粒后发生形态转化的发生率，显著高于将其暴露于类似的无定形NiS颗粒后的发生率。这些效力上的差异归因于结晶态α-NiS颗粒被细胞选择性吞噬，因为未经处理的无定形NiS颗粒不易被摄取。用LiAlH4对无定形NiS颗粒的表面进行化学还原，导致中国仓鼠卵巢（CHO）细胞对其吞噬摄取增加，以及它们诱导SHE细胞转化的能力增强。LiAlH4还原也增强了结晶态α-NiS颗粒的吞噬作用和转化活性。这些结果与先前的观察结果一致，即未经处理的结晶态NiS颗粒具有负表面电荷，而无定形NiS颗粒具有带正电荷的表面。这些发现支持了一个普遍的假设，即颗粒状金属化合物的转化活性与其吞噬摄取成正比。具体而言，这些观察结果表明，金属硫化物颗粒进入细胞与其表面性质有关，特别是与表面微环境上的负电荷程度有关。
