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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Eur J Biochem. 1982 Feb;122(2):333-7. doi: 10.1111/j.1432-1033.1982.tb05885.x.

Cholera toxin has been labelled with the fluorescent probe 4-chloro-7-nitrobenzofuran (Nbf-Cl) in both subunits, and the labelled subunits separated by gel-permeation. They retained their biological activities. 2. Addition of ganglioside GM1 (which binds to subunit B only) to either labelled subunit did not alter the fluorescence of the Nbf probe. 3. Whole toxin was reconstituted using labelled subunit A and unlabelled subunit B. Addition of ganglioside GM1 to the reconstituted toxin enhanced the fluorescence by about 90%, but did not change the wavelength. This enhancement reached a maximum when the ganglioside to toxin ratio was about 1 to 1. Ganglioside GD1b (which does not bind) did not affect the fluorescence. 4. These results suggest that the binding of ganglioside to subunit B alters the environment of the Nbf probe bound to subunit A, presumably by a conformational change.

霍乱毒素的两个亚基均已用荧光探针4-氯-7-硝基苯并呋喃（Nbf-Cl）进行标记，标记后的亚基通过凝胶渗透法分离。它们保留了生物活性。2. 向任一标记亚基中添加仅与亚基B结合的神经节苷脂GM1，不会改变Nbf探针的荧光。3. 使用标记的亚基A和未标记的亚基B重构完整毒素。向重构毒素中添加神经节苷脂GM1可使荧光增强约90%，但不改变波长。当神经节苷脂与毒素的比例约为1比1时，这种增强达到最大值。不结合的神经节苷脂GD1b不影响荧光。4. 这些结果表明，神经节苷脂与亚基B的结合可能通过构象变化改变了与亚基A结合的Nbf探针的环境。

Eur J Biochem. 1982 Feb;122(2):333-7. doi: 10.1111/j.1432-1033.1982.tb05885.x.

Cholera toxin has been labelled with the fluorescent probe 4-chloro-7-nitrobenzofuran (Nbf-Cl) in both subunits, and the labelled subunits separated by gel-permeation. They retained their biological activities. 2. Addition of ganglioside GM1 (which binds to subunit B only) to either labelled subunit did not alter the fluorescence of the Nbf probe. 3. Whole toxin was reconstituted using labelled subunit A and unlabelled subunit B. Addition of ganglioside GM1 to the reconstituted toxin enhanced the fluorescence by about 90%, but did not change the wavelength. This enhancement reached a maximum when the ganglioside to toxin ratio was about 1 to 1. Ganglioside GD1b (which does not bind) did not affect the fluorescence. 4. These results suggest that the binding of ganglioside to subunit B alters the environment of the Nbf probe bound to subunit A, presumably by a conformational change.

霍乱毒素的两个亚基均已用荧光探针4-氯-7-硝基苯并呋喃（Nbf-Cl）进行标记，标记后的亚基通过凝胶渗透法分离。它们保留了生物活性。2. 向任一标记亚基中添加仅与亚基B结合的神经节苷脂GM1，不会改变Nbf探针的荧光。3. 使用标记的亚基A和未标记的亚基B重构完整毒素。向重构毒素中添加神经节苷脂GM1可使荧光增强约90%，但不改变波长。当神经节苷脂与毒素的比例约为1比1时，这种增强达到最大值。不结合的神经节苷脂GD1b不影响荧光。4. 这些结果表明，神经节苷脂与亚基B的结合可能通过构象变化改变了与亚基A结合的Nbf探针的环境。