猪生长激素的异质性。

Heterogeneity of porcine somatotropin.

作者信息

Schleyer M, Trah T, Schleyer I

出版信息

Hoppe Seylers Z Physiol Chem. 1982 Feb;363(2):179-85. doi: 10.1515/bchm2.1982.363.1.179.

DOI:10.1515/bchm2.1982.363.1.179

Abstract

A chromatographical method is presented by which highly purified porcine somatotropin can be separated preparatively into five subfractions. The rechromatographed subfractions show comparable biological activities and molecular sizes, but they differ characteristically in the polyacrylamide gel electrophoresis and in the isoelectric focusing. Relative small differences are found in the amino acid composition of these fractions. The question is discussed, whether the term multiple forms or isohormones of porcine somatotropin is suitable for the growth promoting fractions.

摘要

本文介绍了一种色谱方法，通过该方法可将高度纯化的猪生长激素制备性分离成五个亚组分。重新进行色谱分析的亚组分显示出相当的生物活性和分子大小，但它们在聚丙烯酰胺凝胶电泳和等电聚焦方面具有特征性差异。在这些组分的氨基酸组成中发现了相对较小的差异。文中讨论了“猪生长激素的多种形式”或“同工激素”这一术语是否适用于具有促生长作用的组分这一问题。