Takemoto L J, Hansen J S
Invest Ophthalmol Vis Sci. 1982 Mar;22(3):336-42.
Two-dimensional diagonal electrophoresis has been used to characterize intermolecular disulfide bonding of membrane proteins from lenses of cataractous and normal patients. A component of approximately 18,000 daltons, linked via intermolecular disulfide bonding, was found in membrane preparations from 10 of 17 cataracts studied. In comparison, membrane from only 1 of 12 normal lenses of approximately the same age range was found to contain intermolecular disulfide bonding of a component of similar molecular weight. Treatment of normal lens with the oxidizing agents cupric sulfate and 1,10-phenanthroline resulted in intermolecular disulfide bonding of the 18K component in a manner similar to that found in cataractous lenses. Together these results demonstrate that human cataractogenesis is many times accompanied by intermolecular disulfide bonding of a membrane component of 18K and suggest that this intermolecular bonding may be the result of the previously reported oxidative insult of the lens during human cataract formation.
二维对角线电泳已被用于表征白内障患者和正常患者晶状体膜蛋白的分子间二硫键结合情况。在研究的17例白内障患者中的10例的膜制剂中发现了一种通过分子间二硫键连接的约18,000道尔顿的成分。相比之下，在年龄范围大致相同的12例正常晶状体中，只有1例的膜含有分子量相似的成分的分子间二硫键结合。用氧化剂硫酸铜和1,10-菲咯啉处理正常晶状体，会导致18K成分发生分子间二硫键结合，其方式与在白内障晶状体中发现的类似。这些结果共同表明，人类白内障形成过程中多次伴随着18K膜成分的分子间二硫键结合，并表明这种分子间结合可能是先前报道的人类白内障形成过程中晶状体氧化损伤的结果。
