Acamovic T, D'Mello J P, Fraser K W
J Chromatogr. 1982 Feb 5;236(1):169-79. doi: 10.1016/s0021-9673(00)82510-8.
The estimation of mimosine and 3-hydroxy-4(1H)-pyridone in Leucaena leucocephala, Leucaena seeds, chick excreta and chick serum using reversed-phase ion-pair high-performance liquid chromatography was investigated. Isocratic elution of both compounds was achieved in 11 min using sodium octyl sulphate as the pairing agent in a pH 2.25 buffer. Good recoveries of both mimosine and 3-hydroxy-4(1H)-pyridone in all but serum samples were obtained.