Stuart M C, Underwood P A, Boscato L
J Clin Endocrinol Metab. 1982 May;54(5):881-4. doi: 10.1210/jcem-54-5-881.
Monoclonal antibodies directed toward human PRL (hPRL) have been produced by fusion of mouse myeloma cells (Sp2/0-Ag 14) with spleen cells from mice immunized with hPRL. Total immunizing doses of 20 microgram and 64 microgram hPRL resulted in the production of three highly specific hPRL antibodies. The high affinity antibody, with a Ka value of 0.23 X 10(10) M-1, was used to establish a RIA highly suitable for the measurement of hPRL levels in human serum. The correlation of serum hPRL levels measured using the antibody and those in a conventional rabbit anti-hPRL assay was 0.99 (y = 1.16 - 7.2). These results demonstrate that using the mouse hybridoma technique, it is possible to produce high affinity monospecific monoclonal antibody suitable for the measurement of hPRL in human serum.
通过将小鼠骨髓瘤细胞（Sp2/0-Ag 14）与用人类催乳素（hPRL）免疫的小鼠脾细胞融合，制备了针对人催乳素（hPRL）的单克隆抗体。20微克和64微克hPRL的总免疫剂量产生了三种高度特异性的hPRL抗体。亲和力高的抗体，其Ka值为0.23×10（10）M-1，用于建立一种非常适合测量人血清中hPRL水平的放射免疫分析（RIA）。使用该抗体测量的血清hPRL水平与传统兔抗hPRL分析中的水平的相关性为0.99（y = 1.16 - 7.2）。这些结果表明，使用小鼠杂交瘤技术，可以产生适用于测量人血清中hPRL的高亲和力单特异性单克隆抗体。
