Jones R G, Davis W L, Vinson S B
J Histochem Cytochem. 1982 Apr;30(4):293-304. doi: 10.1177/30.4.7061827.
Potassium pyroantimonate histochemistry, coupled with ethyleneglycoltetraacetic acid (EGTA)-chelation and X-ray microprobe analysis, was employed to localize intracellular calcium binding sites in the normal and degenerating flight musculature in queens of Solenopsis, the fire ant. In normal animals, calcium distribution was light to moderate within myofibrils and mitochondria. In the early contracture stages of the insemination-induced degeneration, both myofilament and mitochondrial calcium loading was markedly increased. In the terminal stages of myofibril breakdown, only Z-lines (isolated or in clusters) with an associated filamentous residue persisted. These complexes were also intensely calcium positive. This study further documents the presence of increased sarcoplasmic calcium during muscle necrosis. Surface membrane defects, mitochondrial calcium overload, and calcium-activated proteases may all be involved in this "normal" breakdown process.
焦锑酸钾组织化学，结合乙二醇四乙酸（EGTA）螯合和X射线微探针分析，用于定位红火蚁（Solenopsis）蚁后正常和退化飞行肌肉组织中的细胞内钙结合位点。在正常动物中，钙在肌原纤维和线粒体中的分布为轻度至中度。在受精诱导的退化早期挛缩阶段，肌丝和线粒体的钙负荷均显著增加。在肌原纤维分解的末期，仅残留有相关丝状残留物的Z线（孤立或成簇）持续存在。这些复合物的钙染色也呈强阳性。本研究进一步证明了肌肉坏死期间肌浆钙增加的存在。表面膜缺陷、线粒体钙超载和钙激活蛋白酶可能都参与了这个“正常”的分解过程。
