Okuda Y, Okamura H, Kanzaki H, Takenaka A, Morimoto K, Nishimura T
Nihon Sanka Fujinka Gakkai Zasshi. 1982 Feb;34(2):181-6.
In the previous study, using carbon tracer, we reported the ultrastructural evidences which suggested the increased capillary permeability of preovulatory rabbit ovarian follicles. In the present study, another tracer, horseradish peroxidase (HRP 50 A in diameter, mw. 40,000), being smaller than carbon particle, was used to examine the permeability of perifollicular capillaries. HRP left capillaries and appeared in the follicular cavity immediately after the injection into aorta, through all stages of follicular development. In the capillaries, the narrow interendothelial clefts (about 100-200 A in width) were observed through preovulatory stages. Fenestrations were noted at 3,4,6 hours after the hCG injection, and interendothelial gaps (about 1,000-10,000 A in width) were seen at 10, 12 hours. HRP passed through these structures and infiltrated the capillary basal lamina and the basal lamina of the follicle and went through the intercellular spaces between granulosa cells. Therefore, the increased permeability of perifollicular capillaries, just prior to ovulation, were not so obvious as when carbon particles were used. On the other hand, carbon particles, injected via marginal ear vein, were incorporated in the endothelial vesicles 15 min. after its administration and retained beneath the basal lamina outside the follicle within 60 min. These observations indicated that "blood follicle barrier" consisting of capillary endothelium, basal lamina, basal lamina of the follicle and granulosa layer played an inhibitory role to large molecules but not to small molecules.
在先前的研究中，我们使用碳示踪剂报道了超微结构证据，表明排卵前兔卵巢卵泡的毛细血管通透性增加。在本研究中，使用了另一种示踪剂辣根过氧化物酶（直径50 Å，分子量40,000），其比碳颗粒小，用于检测卵泡周围毛细血管的通透性。将HRP注入主动脉后，在卵泡发育的各个阶段，HRP均离开毛细血管并立即出现在卵泡腔内。在毛细血管中，在排卵前阶段观察到狭窄的内皮间隙（宽度约100 - 200 Å）。在注射hCG后3、4、6小时观察到窗孔，在10、12小时观察到内皮间隙（宽度约1000 - 10000 Å）。HRP穿过这些结构，渗透到毛细血管基膜和卵泡基膜，并穿过颗粒细胞之间的细胞间隙。因此，排卵前卵泡周围毛细血管通透性的增加并不像使用碳颗粒时那么明显。另一方面，经耳缘静脉注射的碳颗粒在给药后15分钟被内皮小泡摄取，并在60分钟内保留在卵泡外基膜下方。这些观察结果表明，由毛细血管内皮、基膜、卵泡基膜和颗粒层组成的“血卵泡屏障”对大分子起抑制作用，但对小分子不起作用。
