Okuda Y, Okamura H, Kanzaki H, Takenaka A, Morimoto K, Nishimura T
Nihon Sanka Fujinka Gakkai Zasshi. 1980 Jun;32(6):739-48.
The observations that perifollicular capillaries of the ovary become engorged with prominent edema in the theca interna and externa around the time of ovulation, suggest a preovulatory increase in permeability of perifollicular capillaries. In order to obtain an ultrastructural evidence for this increased capillary permeability, we examined the perifollicular capillaries in the theca interna by using mature rabbit ovaries at various time intervals after the hCG injection for induction of ovulation. We observed a capillary engorgement at 9, 10, 12 hours after the hCG injection, most prominent at 9 hours, and edema in the pericapillary space at 10, 12 hours. Pinocytotic vesicles were seen through the all stages of follicular development and they did not show any significant changes in their size, number and distribution. Fenestrations were observed at 0, 3, 4, 6 hours after the hCG injection, most frequent at 4 hours. Intercellular gaps (500 approximately 1,200 A) between two endothelial cells were demonstrated at 10, 12 hours, and thrombocytes in these gaps were also noticed. The present observations suggest that increased permeability of perifollicular capillaries just prior to ovulation were mainly due to the formation of gaps between the endothelial cells.
在排卵前后，卵巢的卵泡周围毛细血管会充血，卵泡膜内外出现明显水肿，这表明卵泡周围毛细血管在排卵前通透性增加。为了获得这种毛细血管通透性增加的超微结构证据，我们使用经人绒毛膜促性腺激素（hCG）注射诱导排卵后的成熟兔卵巢，在不同时间间隔检查卵泡膜内的卵泡周围毛细血管。我们观察到，在注射hCG后9、10、12小时出现毛细血管充血，9小时时最为明显，在10、12小时时毛细血管周围间隙出现水肿。在卵泡发育的各个阶段均可见到吞饮小泡，其大小、数量和分布均无明显变化。在注射hCG后0、3、4、6小时观察到窗孔，4小时时最为频繁。在10、12小时时可见两个内皮细胞之间的细胞间隙（约500至1200埃），并且在这些间隙中也发现了血小板。目前的观察结果表明，排卵前卵泡周围毛细血管通透性增加主要是由于内皮细胞之间形成了间隙。
