Brammer M J, Ray P
J Neurochem. 1982 May;38(5):1493-7. doi: 10.1111/j.1471-4159.1982.tb07931.x.
Various cryoprotective agents [glycerol, dimethylsulphoxide, polyvinylpyrrolidone (PVP 40)], all dissolved in Krebs phosphate medium were tested for their effects on cytoplasmic preservation in oligodendroglia isolated from bovine white matter sorted at -30 degrees C. Of these agents, only PVP 40 (15% wt/vol) produced a significant improvement in recovery of oligodendroglial cytoplasm compared with untreated frozen brain. Cels isolated after PVP 40 pretreatment contained levels of membrane-bound enzymes similar to those found in cells isolated from fresh white matter. There was however, some loss of soluble protein. Studies of galactocerebroside synthesis in neuronal and oligodendroglial perikarya have shown that the glial cells contain ceramide galactosyltransferase at much higher specific activity than the neurones.
各种冷冻保护剂[甘油、二甲基亚砜、聚乙烯吡咯烷酮（PVP 40）]均溶解于磷酸 Krebs 培养基中，测试它们对在 -30℃分选的牛白质中分离出的少突胶质细胞胞质保存的影响。在这些试剂中，与未经处理的冷冻脑相比，只有 PVP 40（15%重量/体积）在少突胶质细胞胞质恢复方面产生了显著改善。PVP 40 预处理后分离的细胞所含膜结合酶水平与从新鲜白质中分离的细胞相似。然而，存在一些可溶性蛋白质的损失。对神经元和少突胶质细胞胞体中半乳糖脑苷脂合成的研究表明，胶质细胞所含神经酰胺半乳糖基转移酶的比活性比神经元高得多。
