Sekardi L, Friedberg K D
Pharmacology. 1982;24(1):45-56. doi: 10.1159/000137575.
A simplified method for the extraction and isolation of histamine from blood, plasma and freeze-dried tissue is described. Extraction is carried out with absolute or 80% ethanol. Subsequently histamine is isolated from an ion-exchange column and determined fluorometrically. The normal histamine contents of blood, plasma and various organs from guinea pigs are investigated by this procedure. The values obtained are compared with the data in the literature. On comparison of the histamine contents in the organs of guinea pigs of two weight and age classes, significant differences are found in the lungs, heart, spleen, kidneys and stomach. There seems to be an inverse relationship between body weight and histamine concentration.
本文描述了一种从血液、血浆和冻干组织中提取和分离组胺的简化方法。用无水乙醇或80%乙醇进行提取。随后，从离子交换柱中分离出组胺并进行荧光测定。通过该方法研究了豚鼠血液、血浆和各种器官中的正常组胺含量。将获得的值与文献中的数据进行比较。比较两个体重和年龄组豚鼠器官中的组胺含量，发现肺、心脏、脾脏、肾脏和胃存在显著差异。体重与组胺浓度之间似乎存在反比关系。
