Kier A B, Schroeder F
Transplantation. 1982 Mar;33(3):274-9. doi: 10.1097/00007890-198203000-00013.
The capacity of LM cells to initiate tumor formation in vivo was investigated in athymic (nude) BALB/c mice. Tumor cells inoculated s.c. over the left thighs of mice formed large, grossly visible tumors within 2 weeks postinoculation. Tumor doubling times were slightly longer than doubling times of LM cells in suspension culture. Histological examination of necropsied mice revealed that tumor cells were invasive into host tissue and formed numerous bizarre mitotic figures. The tumors were classified a fibrosarcoma-like tumors. Metastasis to the lungs was observed in nude mice inoculated with a variety of tumor cells. LM cells cultured in the presence of choline analogues (N,N'-dimethylethanolamine, N-monomethylethanolamine, or ethanolamine) had grossly altered membrane lipid compositions. However, all formed tumors in the nude mice with tumor volume doubling times similar to that of LM cells grown in choline medium. LM cells grown with 10% calf serum had doubling times about 42% shorter. LM fibroblasts injected into nude mice provide a model system for investigation of tumor biology related to metastasis formation.
在无胸腺（裸）BALB/c小鼠中研究了LM细胞在体内引发肿瘤形成的能力。接种于小鼠左大腿皮下的肿瘤细胞在接种后2周内形成了肉眼可见的大肿瘤。肿瘤倍增时间略长于悬浮培养中LM细胞的倍增时间。对剖检小鼠的组织学检查显示，肿瘤细胞侵入宿主组织并形成了许多怪异的有丝分裂图像。这些肿瘤被归类为纤维肉瘤样肿瘤。在接种了多种肿瘤细胞的裸鼠中观察到了肺转移。在胆碱类似物（N，N'-二甲基乙醇胺、N-单甲基乙醇胺或乙醇胺）存在下培养的LM细胞，其膜脂质组成发生了显著改变。然而，所有这些细胞在裸鼠中都形成了肿瘤，其肿瘤体积倍增时间与在胆碱培养基中生长的LM细胞相似。在含10%小牛血清的条件下生长的LM细胞，其倍增时间缩短了约42%。注射到裸鼠体内的LM成纤维细胞为研究与转移形成相关的肿瘤生物学提供了一个模型系统。
