Bienvenu J, Bienvenu F, Michaud-Caudie C, Quincy C, Nicolas J P, Phang P P, Mandrand B, Trabuc-Jaubert M
Ann Clin Biochem. 1982 Jan;19(Pt 1):61-3.
We report an evaluation of a commercially available kit, the Biomérieux IgE-kit, for determination of total IgE in serum by the EIA double antibody sandwich method. Results by this procedure and PRIST (Pharmacia) show a degree of association (r) of 0.98 and a regression equation of log Y = 0.79 log PRIST + log 3.31. Correlation between the IgE kit and RIST shows a degree of association of 1.00 and a regression equation of log Y = 1.01 log RIST--log 1.05. Between-laboratory standard deviations are 3%, 3%, and 15% for the IgE concentrations of, respectively, 83, 285, and 900 IU/ml. Similarly, intra- and inter-assay correlations were performed on 60 lyophilised serum samples tested in the four centres. No protein interference was found except for cryoprecipitates. The favourable correlation with existing procedures and the feasibility of this kit offer a new way of measuring IgE levels.
我们报告了一项对市售试剂盒（生物梅里埃IgE试剂盒）的评估，该试剂盒采用酶免疫分析双抗体夹心法测定血清中的总IgE。通过该方法和PRIST（法玛西亚公司产品）得到的结果显示，二者的关联度（r）为0.98，回归方程为log Y = 0.79 log PRIST + log 3.31。IgE试剂盒与RIST之间的相关性显示，关联度为1.00，回归方程为log Y = 1.01 log RIST - log 1.05。对于浓度分别为83、285和900 IU/ml的IgE，实验室间标准差分别为3%、3%和15%。同样，对在四个中心检测的60份冻干血清样本进行了批内和批间相关性分析。除冷沉淀物外，未发现蛋白质干扰。该试剂盒与现有方法的良好相关性及其可行性为测量IgE水平提供了一种新方法。
