Komarov E V, Oleĭnikova G A, Seleznev A P, Serova I P, Apter Iu M
Antibiotiki. 1982 Feb;27(2):103-5.
Marketed oleandomycin phosphate, as well as the International Standard of oleandomycin (Great Britain, the 1st Int. Std., 1964) were studied with a purpose of determining their composition. The components were separated by liquid chromatography on columns with Sefadex LH-20 using 50 per cent ethanol as an eluent. The Lowry method using the biuret test and assays of the amino acids in the hydrolysates were used to show for the first time that marketed oleandomycin and its International Standard contained an admixture of the peptide nature. The data of the amino acid assays suggest that the admixture and oleandomycin is a fixed complex (adduct).
对市售磷酸竹桃霉素以及竹桃霉素国际标准品（英国，1964年第一国际标准品）进行了研究，目的是确定其成分。使用50%乙醇作为洗脱剂，通过在装有葡聚糖凝胶LH - 20的柱上进行液相色谱法分离各成分。首次使用基于双缩脲试验的洛瑞法以及水解产物中氨基酸的测定方法，以表明市售竹桃霉素及其国际标准品含有肽性质的混合物。氨基酸测定数据表明，该混合物与竹桃霉素是一种固定的复合物（加合物）。
