射出的黑猩猩精子的表面成分。

Surface components of ejaculated chimpanzee sperm.

作者信息

Young L G, Gould K G

出版信息

Arch Androl. 1982 Feb;8(1):15-20. doi: 10.3109/01485018208987012.

DOI:10.3109/01485018208987012

Abstract

Surface components of ejaculated chimpanzee sperm were radiolabeled by enzymatic iodination with lactoperoxidase and Na125I. The sperm were lysed and the labeled components analyzed on SDS-7.5% polyacrylamide gels. Electrophoresis of solubilized radioactivity resolved four components with approximate molecular weights of 72,000, 49,000, 25,000 and 17,000 daltons. To identify components, which might be adsorbed to the chimpanzee sperm surface from seminal secretions, seminal plasma was labeled. Electrophoresis of labeled seminal plasma resolved three components with approximate molecular weights of 63,000, 26,000, and 17,000 daltons, two of which comigrated with a labeled sperm surface component.

摘要

通过用乳过氧化物酶和Na125I进行酶促碘化，对射出的黑猩猩精子的表面成分进行放射性标记。精子被裂解，标记的成分在SDS - 7.5%聚丙烯酰胺凝胶上进行分析。溶解的放射性物质的电泳分离出四种成分，其近似分子量分别为72,000、49,000、25,000和17,000道尔顿。为了鉴定可能从精液分泌物吸附到黑猩猩精子表面的成分，对精浆进行了标记。标记精浆的电泳分离出三种成分，其近似分子量分别为63,000、26,000和17,000道尔顿，其中两种与标记的精子表面成分迁移率相同。