[放线菌素D与DNA结合过程中“识别”的GC碱基对数量的测定]
[Determination of the number of GC-pairs "recognized" by actinomycin D during binding with DNA].
作者信息
Mikhaĭlov M V, Zasedatelev A S, Gurskiĭ G V
出版信息
Biofizika. 1982 Jan-Feb;27(1):14-6.
PMID:7066384
Abstract
Experimental studies are reported on the binding of actinomycin D to various synthetic and naturally occurring DNAs. The experimental data obtained agree with a model in which the antibiotic molecule carries only one GC-specific reaction center and covers 5 base pairs upon binding to DNA. Positive cooperative effects are observed for the binding of actinomycin to poly(dG-dC)-poly (dG-dC) duplex.
摘要
报道了关于放线菌素D与各种合成DNA和天然DNA结合的实验研究。获得的实验数据与一个模型相符，在该模型中，抗生素分子仅携带一个GC特异性反应中心，并且在与DNA结合时覆盖5个碱基对。观察到放线菌素与聚（dG-dC）-聚（dG-dC）双链体结合存在正协同效应。
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