Oko R, Hrudka F
Biol Reprod. 1982 Feb;26(1):183-95. doi: 10.1095/biolreprod26.1.183.
The effect of gossypol upon organelles of rat sperm was investigated by light and electron microscopy. The drug was administered s.c. for 2 to 30 days at 20 mg/kg BW/day. Sperm from the testis, caput, corpus, and cauda epididymis were examined at regular intervals, during and after treatment, for periods extending up to 70 days. The drug induced a specific effect in the sperm tail. It consisted of segmental aplasia of the mitochondrial sheath observed in high incidence in testicular and epididymal sperm. This primary lesion, in the authors' view, predisposed a development of secondary lesions as sperm advanced along the epididymis. Secondary lesions included bulging, dislocating, fraying, or breaking of axial fibers, bending or breaking of the tail, and decapitation. A minimum of 3 days of treatment was necessary to produce an effect above control values, while 9 days or longer induced lesions in almost all sperm. Motility ceased with 30 days of treatment. Fertilizing capacity was inversely related to the increase and decline of lesions.
通过光学显微镜和电子显微镜研究了棉酚对大鼠精子细胞器的影响。以20mg/kg体重/天的剂量皮下注射该药物，持续2至30天。在治疗期间和治疗后的定期时间点，直至70天，对来自睾丸、附睾头、附睾体和附睾尾的精子进行检查。该药物在精子尾部产生了特定作用。其表现为线粒体鞘节段性发育不全，在睾丸和附睾精子中高发。作者认为，随着精子沿附睾前行，这种原发性病变易引发继发性病变。继发性病变包括轴丝膨出、脱位、磨损或断裂、尾部弯曲或断裂以及断头。至少3天的治疗才能产生高于对照值的效果，而9天或更长时间的治疗几乎会使所有精子出现病变。治疗30天后精子活力停止。受精能力与病变的增加和减少呈负相关。
