Scannell J, Morgan M J
Biosci Rep. 1982 Feb;2(2):99-106. doi: 10.1007/BF01116175.
Chinese-hamster ovary (CHO-K1) cells can grow in medium in which glucose is replaced by either maltose, trehalose, or starch. This growth is due to the presence in the medium of serum enzymes which degrade these substrates to glucose. When the enzymes are inactivated by heat treatment, the cells no longer grow on these substrates but still grow on glucose. Medium containing heat-inactivated serum and the appropriate substrate in place of glucose has been successfully used to isolate starch- and maltose-utilizing variants of CHO-K1 cells.
中国仓鼠卵巢（CHO-K1）细胞能够在葡萄糖被麦芽糖、海藻糖或淀粉替代的培养基中生长。这种生长归因于培养基中存在血清酶，这些酶可将这些底物降解为葡萄糖。当酶通过热处理失活时，细胞不再在这些底物上生长，但仍能在葡萄糖上生长。含有热灭活血清和适当底物以替代葡萄糖的培养基已成功用于分离CHO-K1细胞的淀粉利用变体和麦芽糖利用变体。
