Robinson S I, Nelkin B D, Vogelstein B
Cell. 1982 Jan;28(1):99-106. doi: 10.1016/0092-8674(82)90379-8.
The DNA in a eucaryotic nucleus is arranged into a series of supercoiled loops that are anchored at their bases to the nuclear matrix. Using nuclease digestion, one can progressively cleave DNA from the loops, thereby isolating residual DNA that is progressively closer to the nuclear matrix anchorage sites. We have determined that the ovalbumin gene is preferentially associated with the nuclear matrix of chicken oviduct cells, but is not preferentially associated with the nuclear matrix of chicken liver cells. As a control, the beta-globin gene, which is not transcribed in oviduct cells, was found not to be preferentially associated with the oviduct nuclear matrix. The observation that the transcriptionally active ovalbumin gene is preferentially associated with the nuclear matrix may have significant implications for gene expression and the organization of nuclear DNA into supercoiled-loop domains.
真核细胞核中的DNA被排列成一系列超螺旋环，这些环的基部锚定在核基质上。使用核酸酶消化，可以逐步从环中切割DNA，从而分离出越来越接近核基质锚定位点的残留DNA。我们已经确定，卵清蛋白基因优先与鸡输卵管细胞的核基质相关联，但不优先与鸡肝细胞的核基质相关联。作为对照，发现在输卵管细胞中不转录的β-珠蛋白基因与输卵管核基质没有优先关联。转录活跃的卵清蛋白基因优先与核基质相关联这一观察结果可能对基因表达以及核DNA组织成超螺旋环结构域具有重要意义。
