Miyake M, Morino H, Mizobuchi M, Kakimoto Y
Clin Chim Acta. 1982 Mar 26;120(1):119-26. doi: 10.1016/0009-8981(82)90082-1.
N-Acetyl-L-aspartic acid (NA-Asp), N-acetyl-alpha-L-aspartyl-L-glutamic acid (NA-Asp-Glu) and beta-citryl-L-glutamic acid (beta-CG), which are known to occur in the brain, have been isolated from human urine. Their identities were proved by comparing them with synthetic NA-Asp, NA-Asp-Glu and beta-CG using electrophoretic and chromatographic methods and by acid hydrolysis. A method was developed for the quantitation of NA-Asp, NA-Asp-Glu and beta-CG in human urine. It consists of ion-exchange chromatography followed by gas-chromatographic analysis. The amounts of urinary excretion of NA-Asp, NA-Asp-Glu and beta-CG were 41.2 +/- 10.1 (n = 27), 20.8 +/- 9.6 (n = 27) and 30.2 +/- 13.2 (n = 21) mumol/g creatinine in adult males, and 62.2 +/- 16.3 (n = 27), 24.0 +/- 8.2 (n = 27) and 40.5 +/- 21.1 (n = 24) mumol/g creatinine in adult females, respectively.
已知存在于大脑中的N-乙酰-L-天冬氨酸（NA-天冬氨酸）、N-乙酰-α-L-天冬氨酰-L-谷氨酸（NA-天冬氨酸-谷氨酸）和β-柠檬酰-L-谷氨酸（β-CG）已从人尿中分离出来。通过电泳和色谱方法并经酸水解，将它们与合成的NA-天冬氨酸、NA-天冬氨酸-谷氨酸和β-CG进行比较，从而证明了它们的特性。已开发出一种定量测定人尿中NA-天冬氨酸、NA-天冬氨酸-谷氨酸和β-CG的方法。该方法包括离子交换色谱法，随后进行气相色谱分析。成年男性尿中NA-天冬氨酸、NA-天冬氨酸-谷氨酸和β-CG的排泄量分别为41.2±10.1（n = 27）、20.8±9.6（n = 27）和30.2±13.2（n = 21）μmol/g肌酐，成年女性分别为62.2±16.3（n = 27）、24.0±8.2（n = 27）和40.5±21.1（n = 24）μmol/g肌酐。
