不同白蛋白和动物血清与固定化汽巴蓝的相互作用：表观亲和常数的评估
Interactions of different albumins and animal sera with insolubilized Cibacron Blue, Evaluation of apparent affinity constants.
作者信息
Naval J, Calvo M, Lampreave F, Piñeiro A
出版信息
Comp Biochem Physiol B. 1982;71(3):403-7. doi: 10.1016/0305-0491(82)90401-1.
PMID:7067402
Abstract
- A high concentration Cibacron Blue-Sepharose derivative has been used to study the affinity chromatography of albumin from eight animal species. 2. The apparent affinity constants for albumin varies between 3.9 x 10(4) M-1 and 0.9 x 10(4) M-1, in the order: Human greater than rabbit greater than horse greater than pig = dog greater than bovine greater than rat greater than chicken. 3. Other serum proteins were also bound to the gel, particularly lipoproteins and alpha 2-macroglobulin.
摘要
- 一种高浓度的汽巴蓝-琼脂糖衍生物已被用于研究来自八个动物物种的白蛋白的亲和色谱。2. 白蛋白的表观亲和常数在3.9×10⁴ M⁻¹至0.9×10⁴ M⁻¹之间变化，顺序为：人＞兔＞马＞猪 = 狗＞牛＞大鼠＞鸡。3. 其他血清蛋白也与凝胶结合，特别是脂蛋白和α2-巨球蛋白。
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