Hagmar B, Ryd W
Eur J Nucl Med. 1982;7(3):108-14. doi: 10.1007/BF00256397.
We have reviewed the literature on 125IUDR (5-iodo-2-deoxyuridine), the DNA label of choice for cell distribution studies. In previous studies, the cytotoxicity in relation to labeling efficiency has often been inadequately investigated or reported. We have studied four syngeneic mouse tumours and compared in vitro with in vivo labelling procedures. Ascites tumours could be effectively labelled in vivo by IP injections of 60-80 muCi per mouse during 24 h, without apparent cytotoxicity. Comparable in vitro labelling was also effective, but caused a dose-related cytotoxicity, as measured by growth rate and transplantability. Comparison with 'cold' IUDR disclosed that the toxicity was not chemical, but radiological. Our attempt to label a solid lymphoma in vivo was unsuccessful, obviously because the tumour grows too slowly. We conclude that 125IUDR-labelling is suitable for cell distribution studies, but in view of labelling efficiency versus cytotoxicity the procedure has to be adjusted to each new tumour individually.
我们回顾了有关125IUDR（5-碘-2-脱氧尿苷）的文献，它是细胞分布研究中首选的DNA标记物。在以往的研究中，细胞毒性与标记效率之间的关系常常未得到充分研究或报道。我们研究了四种同基因小鼠肿瘤，并对体外标记程序和体内标记程序进行了比较。腹水肿瘤可通过每只小鼠腹腔注射60 - 80微居里，在24小时内进行有效的体内标记，且无明显细胞毒性。类似的体外标记也有效，但会产生与剂量相关的细胞毒性，这可通过生长速率和移植能力来衡量。与“冷”IUDR比较表明，毒性并非化学性的，而是放射性的。我们尝试对实体淋巴瘤进行体内标记未成功，显然是因为肿瘤生长过于缓慢。我们得出结论，125IUDR标记适用于细胞分布研究，但鉴于标记效率与细胞毒性，该程序必须针对每种新肿瘤单独进行调整。
