Jenkinson I S, Shuter B J, Wright P N
Cell Tissue Kinet. 1975 Jul;8(4):307-20. doi: 10.1111/j.1365-2184.1975.tb01496.x.
Superficial Ehrlich tumours in mice were used to assess how much information on cell kinetics could be obtained from only the simplest techniques of autoradiography, and in situ monitoring of 125I-iodo-deozyuridine. These techniques were selected as being readily applicable to clinical situations. Intradermal tumours were studied from the earliest stages of rapid growth to large slow growing tumours with necrotic cores, as well as tumours undergoing regression. For comparison, intramuscular tumours were studied with systemic injections of radioactive DNA precursors. It was found that extensive information on cell production and loss rates was obtained from a single injection of tritiated thymidine followed by a single biopsy, or alternatively in vitro labelling of one minute biopsy specimen, and measurements of only the labelling index, together with a knowledge of the tumour's growth characteristics. Following a single localized injection of 125I-iodo-deoxyuridine, the loss rate of radioactivity measured in situ for a period of about 1 week provided an index of cell loss rate from the tumours.
利用小鼠体表的艾氏瘤来评估仅通过放射自显影和125I-碘脱氧尿苷原位监测等最简单技术能获取多少细胞动力学信息。选择这些技术是因为它们易于应用于临床情况。研究了皮内肿瘤从快速生长的早期阶段到具有坏死核心的大型缓慢生长肿瘤，以及正在消退的肿瘤。作为比较，通过全身注射放射性DNA前体来研究肌内肿瘤。结果发现，单次注射氚标记胸腺嘧啶核苷后进行单次活检，或者对一分钟活检标本进行体外标记，并仅测量标记指数，同时了解肿瘤的生长特征，就能获得关于细胞产生和丢失率的大量信息。在单次局部注射125I-碘脱氧尿苷后，原位测量约1周时间内的放射性损失率可提供肿瘤细胞丢失率的指标。
