Cullheim S, Risling M
Exp Brain Res. 1982;45(3):428-32. doi: 10.1007/BF01208603.
In 1 week old kittens a 10 mm long segment of the sciatic nerve was removed distal to the hamstring branch point. After 70-85 days sciatic motoneurons were injected intracellularly with horseradish peroxidase (HRP). The cell body diameter, the summed diameter of the 1st order dendrites, the intramedullary axon diameter and the axon conduction velocity proximal to the lesion were determined in individual neurons and related to the conduction velocity of the regenerating segment of the axons. The lesioned neurons were compared with normal sciatic motoneurons in 7-8 and 77-92 days old kittens. In motoneurons which seemed to lack regenerating axons or had regenerating axon segments with a low conduction velocity there was a clear growth retardation of all studied parameters. In those neurons, however, which had regenerating axon segments conducting faster than 10 m/s, all parameters above the lesion, except for the conduction velocity of the axon, had attained normal values. The intramedullary part of the axon seemed to be less affected by the injury than all other studied parts of the neuron proximal to the lesion.
在1周龄的小猫中，在坐骨神经的绳肌分支点远端切除一段10毫米长的神经。70 - 85天后，向坐骨运动神经元内注射辣根过氧化物酶（HRP）。测定单个神经元的细胞体直径、一级树突的总直径、损伤近端的髓内轴突直径和轴突传导速度，并将其与轴突再生段的传导速度相关联。将损伤的神经元与7 - 8日龄和77 - 92日龄小猫的正常坐骨运动神经元进行比较。在似乎缺乏再生轴突或具有低传导速度的再生轴突段的运动神经元中，所有研究参数均明显生长迟缓。然而，在那些具有传导速度快于10米/秒的再生轴突段的神经元中，损伤上方的所有参数，除了轴突传导速度外，均已达到正常值。轴突的髓内部分似乎比损伤近端神经元的所有其他研究部分受损伤的影响更小。
