针叶树（欧洲赤松）的异柠檬酸裂解酶
Isocitrate lyase of conifers (Pinus pinea).
作者信息
Pinzauti G, Giachetti E, Vanni P
出版信息
Int J Biochem. 1982;14(4):267-75. doi: 10.1016/0020-711x(82)90087-8.
PMID:7067906
Abstract
- Isocitrate lyase has been purified about 60 times from the conifer Pinus pinea. A first characterization was made. 2. The high instability is an important feature of this enzyme from higher plants, this causes serious problems in the purification and characterization. 3. A substantial agreement with the data from the literature was found for what concerns pH dependence of Vmax and pKm, the effect of bivalent cations and the requirement of Mg2+. 4. Kinetic studies gave evidence for a mechanism ordered uni-bi with glyoxylate being the last product released, kinetic constants were calculated, no evidence for cooperative effects was found. 5. Equilibrium constant by Haldane method calculation agrees with value calculated with isocitrate lyase from the bacterium Pseudomonas indigofera.
摘要
异柠檬酸裂解酶已从针叶树欧洲赤松中纯化了约60倍。进行了首次表征。
高不稳定性是这种来自高等植物的酶的一个重要特征，这在纯化和表征过程中会引发严重问题。
在Vmax和pKm的pH依赖性、二价阳离子的作用以及对Mg2+的需求方面，发现与文献数据有很大一致性。
动力学研究证明了一种有序的单底物-双底物机制，乙醛酸是最后释放的产物，计算了动力学常数，未发现协同效应的证据。
通过霍尔丹方法计算的平衡常数与用来自产靛假单胞菌的异柠檬酸裂解酶计算的值一致。
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