Hauser S E, Almeida A
ISA Trans. 1982;21(1):31-5.
A microprocess-based instrument has been designed to facilitate research on the electrical properties of isolated renal tubules. Because of the high correlation between the electrical and ionic properties of tubules, this research helps in understanding the mechanisms of reabsorption and secretion along the many functionally different segments of a renal tubule. By replacing manual methods of control, measurement, and calculation, this instrument expedites the experimental procedure and adds accuracy and flexibility not available in the previous arrangement. Based on operational directives entered by the user, the instrument controls the number, frequency, polarity, intensity, and duration of current pulses through the tubule preparation and records the steady-state voltages induced at either end of the tubule. Then, using a transmission line model, it calculates and displays tubule parameters for each current pulse. The diameter of the tubule, as calculated from the model equations, is of immediate interest to the investigator. How well this figure compares with the physical diameter is an indication of the integrity of the preparation and the validity of the other calculations.
一种基于微处理器的仪器已被设计出来，以促进对离体肾小管电特性的研究。由于肾小管的电特性和离子特性之间高度相关，这项研究有助于理解沿肾小管许多功能不同节段的重吸收和分泌机制。通过取代手动控制、测量和计算方法，该仪器加快了实验过程，并增加了以前装置所没有的准确性和灵活性。根据用户输入的操作指令，该仪器控制通过肾小管制剂的电流脉冲的数量、频率、极性、强度和持续时间，并记录在肾小管两端感应的稳态电压。然后，使用传输线模型，它计算并显示每个电流脉冲的肾小管参数。根据模型方程计算出的肾小管直径是研究人员立即感兴趣的。这个数字与实际直径的符合程度表明了制剂的完整性和其他计算的有效性。
