Findlay J K, Clarke I J, Swaney J, Colvin N, Doughton B
J Reprod Fertil. 1982 Mar;64(2):329-39. doi: 10.1530/jrf.0.0640329.
The rate of protein synthesis was higher in caruncular (P less than 0.05) and intercaruncular endometrium (P less than 0.01) of pregnant compared to non-pregnant ewes on Day 11 (Day 0 = day of oestrus), but not on Days 9, 13 and 15. Cytosolic oestrogen receptor concentrations (nmol/mg protein) were lower in caruncular endometrium of pregnant ewes than in non-pregnant ewes on Days 9 (P less than 0.02), 13 (P less than 0.05) and 15 (P less than 0.001) but not on Day 11. In intercaruncular endometrium, the receptor values were lower on Day 15 (P less than 0.02) of pregnancy compared to non-pregnancy, but not on the other days. The dissociation constant (0.75-2.55 x 10(-10) M) of oestradiol-17 beta for cytosolic receptors was similar in both tissues, regardless of pregnancy status or day of sampling. The rate of protein synthesis was generally higher in intercaruncular endometrium whereas cytosol receptor concentrations were higher in caruncular endometrium. When the blastocyst was confined to one uterine horn on Day 2 in another group of ewes, there was a decrease in nuclear (P less than 0.05), cytosolic (P less than 0.05) and total (P less than 0.05) oestrogen receptors only in caruncular endometrium in the pregnant horn on Day 15. There were no difference in the proportions of receptors localized in nuclear fractions or in the rate of protein synthesis in either endometrial tissue from the pregnant or the non-pregnant horn. We conclude that the ovine blastocyst is capable of influencing oestrogen receptors levels in caruncular and intercaruncular endometrium by a local action as early as Day 9, well before endocrine recognition of pregnancy on Day 12 and attachment on Day 15.
在第11天（第0天=发情日），与未怀孕母羊相比，怀孕母羊子宫阜（P<0.05）和子宫阜间子宫内膜（P<0.01）的蛋白质合成速率更高，但在第9、13和15天则不然。在第9天（P<0.02）、13天（P<0.05）和15天（P<0.001），怀孕母羊子宫阜子宫内膜的胞质雌激素受体浓度（nmol/mg蛋白质）低于未怀孕母羊，但在第11天没有差异。在子宫阜间子宫内膜中，与未怀孕相比，怀孕第15天的受体值较低（P<0.02），但在其他天数没有差异。无论怀孕状态或取样日如何，两种组织中雌二醇-17β与胞质受体的解离常数（0.75 - 2.55×10⁻¹⁰M）相似。子宫阜间子宫内膜的蛋白质合成速率通常较高，而子宫阜子宫内膜的胞质受体浓度较高。在另一组母羊中，当第2天胚泡局限于一个子宫角时，仅在怀孕角的子宫阜子宫内膜中，第15天核（P<0.05）、胞质（P<0.05）和总（P<0.05）雌激素受体减少。怀孕或未怀孕角的任何一种子宫内膜组织中，位于核部分的受体比例或蛋白质合成速率均无差异。我们得出结论，早在第9天，绵羊胚泡就能够通过局部作用影响子宫阜和子宫阜间子宫内膜中的雌激素受体水平，这远早于第12天的内分泌性妊娠识别和第15天的着床。
