Reeve W J
J Reprod Immunol. 1982 Feb;4(1):53-64. doi: 10.1016/0165-0378(82)90023-7.
Culture of precompact zona-free mouse embryos in the plant lectin concanavalin A (Con A) at 20 micrograms/ml inhibits blastocyst formation. Cell division is retarded compared with control embryos, compaction of the morula is prevented, and fluid accumulation occurs within but not between cells. As little as two hours of incubation in Con A can cause developmental delay and an eventual increased incidence of lysis, and although embryos can recover from more prolonged pulses of Con A, the blastocysts formed are often abnormal. Blastocyst formation occurs in some embryos, but does not do so unless at least some cells have undergone a fifth round of cell division before incubation in the lectin. However, not all embryos that contained more than 32 cells compacted and showed intercellular fluid accumulation in Con A. Thus, blastocyst formation is not restricted solely by an inhibition of cell division or a reduction in cell number, but is also impaired by interference of the lectin with the cell surface and its interactions. Morulae bind divalent Con A and monovalent succinyl-Con A similarly, but developmental interference is absent with the latter, suggesting that divalent Con A acts through a cross-linking of surface receptors.
将无透明带小鼠前致密化胚胎置于浓度为20微克/毫升的植物凝集素伴刀豆球蛋白A（Con A）中培养会抑制囊胚形成。与对照胚胎相比，细胞分裂受到抑制，桑椹胚的致密化过程被阻止，并且细胞内而非细胞间出现液体聚集。在Con A中孵育仅两小时就会导致发育延迟并最终增加裂解发生率，尽管胚胎可以从更长时间的Con A脉冲处理中恢复，但形成的囊胚通常是异常的。一些胚胎会发生囊胚形成，但除非至少有一些细胞在凝集素中孵育前经历了第五轮细胞分裂，否则不会发生囊胚形成。然而，并非所有含有超过32个细胞的胚胎在Con A中都会致密化并出现细胞间液体聚集。因此，囊胚形成不仅受到细胞分裂抑制或细胞数量减少的限制，还受到凝集素对细胞表面及其相互作用的干扰的损害。桑椹胚与二价Con A和单价琥珀酰-Con A的结合相似，但后者不存在发育干扰，这表明二价Con A通过表面受体的交联起作用。
