Thompson S M, Suzuki Y, Schultz S G
J Membr Biol. 1982;66(1):55-61. doi: 10.1007/BF01868481.
In this paper we employ the data described in the previous paper (I) to derive the current-voltage (I-V) relations of the basolateral membrane, the amiloride-insensitive "leak" pathway across the apical membrane, and the parallel pathways across rabbit descending colon. The results indicated that: a) The resistance of the basolateral membrane is independent of the electrical potential difference across that barrier over the range -8 to 67 mV and averaged 195 omega cm2. The electromotive force across this barrier averaged 50 mV under control conditions and 48 mV in the presence of amiloride. The origin of this difference is discussed. b) The resistance of the parallel pathways averaged 351 omegacm2 and was independent of the transepithelial electrical potential difference over the range -170 to + 90mV. The conductance of these pathways can be reasonably well accounted for by the partial ionic conductances of Na, K and Cl reported previously. c) The resistance of the amiloride-insensitive pathway across the apical membrane averaged 1667 omegacm2 and the electromotive force across this pathway averaged -51 mV. These values are in excellent agreement with those determined by others. The ionic nature of this "leak" pathway remains to be elucidated.
在本文中，我们利用上一篇论文（I）中描述的数据，来推导基底外侧膜的电流-电压（I-V）关系、穿过顶端膜的amiloride不敏感“渗漏”途径以及兔降结肠的平行途径。结果表明：a）基底外侧膜的电阻在-8至67 mV范围内与跨该屏障的电势差无关，平均为195Ω·cm²。在对照条件下，跨此屏障的电动势平均为50 mV，在amiloride存在时为48 mV。讨论了这种差异的来源。b）平行途径的电阻平均为351Ω·cm²，并且在-170至 + 90mV范围内与跨上皮电势差无关。这些途径的电导可以通过先前报道的Na、K和Cl的部分离子电导得到合理的解释。c）穿过顶端膜的amiloride不敏感途径的电阻平均为1667Ω·cm²，跨此途径的电动势平均为-51 mV。这些值与其他人测定的值非常一致。这种“渗漏”途径的离子性质仍有待阐明。
