Larson B R
Life Sci. 1982 Mar 22;30(12):1003-12. doi: 10.1016/0024-3205(82)90518-5.
Presented is a pilot project describing a new strategy for mass spectrometric peptide sequencing. Using orthophthalaldehyde (OPA), two peptides were derivatized and their fluorescent adducts isolated using reversed phase high performance liquid chromatography. The OPA-peptides were permethylated and the derivatized molecules subjected to direct probe mass spectral analysis. The spectra obtained from OPA-peptides was analogous to those observed for standard N-acyl permethyl derivatives enabling the complete sequence of the peptides to be determined.
本文介绍了一个试点项目，该项目描述了一种用于质谱肽测序的新策略。使用邻苯二甲醛（OPA）对两种肽进行衍生化，并使用反相高效液相色谱法分离其荧光加合物。将OPA-肽进行全甲基化，并对衍生化分子进行直接探针质谱分析。从OPA-肽获得的光谱与标准N-酰基全甲基衍生物观察到的光谱相似，从而能够确定肽的完整序列。
