促阿片黑素细胞皮质素片段对肾上腺嗜铬细胞儿茶酚胺释放的影响。

Effects of pro-opiomelanocortin fragments on release of catecholamines from adrenal chromaffin cells.

作者信息

Boksa P, Seidah N G, Chrétien M, Livett B G

出版信息

Neurosci Lett. 1982 Feb 12;28(2):199-204. doi: 10.1016/0304-3940(82)90152-5.

Abstract

The effects of pro-opiomelanocortin (POMC) peptide fragments on the basal and agonist-induced release of catecholamines (CAs) from monolayer cultures of purified bovine adrenal chromaffin cells were tested. None of the 5 peptides tested, i.e. beta-MSH, ACTH1-39, gamma-MSH1-13, gamma 3-MSH and N-terminal POMC fragment, had any effect on basal CA release. However, beta-MSH (10(-5) M), gamma-MSH1-13 (10(-6)-10(-5) M), gamma 3-MSH (10(-5) M) and N-terminal POMC fragment (10(-5) M) inhibited the nicotine-induced release of CAs from the chromaffin cells. The possible physiological significance of this inhibitory neuromodulation is discussed.

摘要

检测了促阿片-黑素细胞皮质素原（POMC）肽片段对纯化的牛肾上腺嗜铬细胞单层培养物中儿茶酚胺（CA）基础释放及激动剂诱导释放的影响。所检测的5种肽，即β-促黑素（β-MSH）、促肾上腺皮质激素1-39（ACTH1-39）、γ-促黑素1-13（γ-MSH1-13）、γ3-促黑素（γ3-MSH）和POMC N端片段，均对CA基础释放无任何影响。然而，β-MSH（10⁻⁵ M）、γ-MSH1-13（10⁻⁶ - 10⁻⁵ M）、γ3-MSH（10⁻⁵ M）和POMC N端片段（10⁻⁵ M）抑制了嗜铬细胞中尼古丁诱导的CA释放。讨论了这种抑制性神经调节的可能生理意义。