Kaibara K, Koketsu K, Akasu T, Miyagawa M
Pflugers Arch. 1982 Jan;392(3):304-6. doi: 10.1007/BF00584316.
The 22Na+-efflux from skeletal muscle cells of frog (Rana nigromaculata) was measured in Ringer solutions containing different concentrations of K+ (0.1 to 30 mM). The effects of adrenaline (30 microM) and ouabain (10 microM) on the 22Na+-efflux were investigated for the purpose to clarify the mechanism of the facilitatory effect of adrenaline on Na+ - K+ pump. The rate coefficient for the ouabain-sensitive 22 Na+-efflux increases with increasing extracellular K+ concentrations and adrenaline potently facilitates these rate coefficients. On the basis of Michaelis-Menten type kinetics assumed for the reaction between pump site and extracellular K+, it is concluded that adrenaline decreases the dissociation constant (Km), and increases the maximum Na+-efflux.
在含有不同浓度钾离子（0.1至30 mM）的林格氏液中，测定了黑斑蛙骨骼肌细胞的22Na+流出量。为了阐明肾上腺素对钠钾泵促进作用的机制，研究了肾上腺素（30 microM）和哇巴因（10 microM）对22Na+流出量的影响。哇巴因敏感的22Na+流出速率系数随细胞外钾离子浓度的增加而增加，肾上腺素能显著促进这些速率系数。基于泵位点与细胞外钾离子反应的米氏动力学模型，得出肾上腺素降低解离常数（Km）并增加最大钠流出量的结论。
