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双链DNA中极性肽对碱基对的识别

Recognition of base pairs by polar peptides in double stranded DNA.

作者信息

Laigle A, Chinsky L, Turpin P Y

出版信息

Nucleic Acids Res. 1982 Mar 11;10(5):1707-20. doi: 10.1093/nar/10.5.1707.

Abstract

The resonance Raman spectrum of native DNA has been obtained using excitation at 257 nm. In a first part, the spectral lines are assigned to the different nucleotide bases which provide the resonance effect. In a second part, the interactions of DNA with basic peptides (Arginine Methylester, Lysine Methylester, Arginyl-Arginine) are investigated using excitation at 300 nm and 257 nm, which give complementary information about the DNA. Both Arginine Methylester and Arginyl-arginine recognize the A-T base pairs, the first one in the large groove, the second one in the narrow groove of DNA. The DNA-Lysine Methylester interaction is very likely not specific but can take place in the large groove of DNA.

摘要

利用257nm的激发光获得了天然DNA的共振拉曼光谱。在第一部分中,光谱线被归属于提供共振效应的不同核苷酸碱基。在第二部分中,使用300nm和257nm的激发光研究了DNA与碱性肽(精氨酸甲酯、赖氨酸甲酯、精氨酰 - 精氨酸)的相互作用,这两种激发光给出了关于DNA的互补信息。精氨酸甲酯和精氨酰 - 精氨酸都能识别A - T碱基对,前者在DNA的大沟中识别,后者在窄沟中识别。DNA - 赖氨酸甲酯的相互作用很可能是非特异性的,但可发生在DNA的大沟中。

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本文引用的文献

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Interactions of oligopeptides with nucleic acids.寡肽与核酸的相互作用。
CRC Crit Rev Biochem. 1981;10(3):213-58. doi: 10.3109/10409238109113600.

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