Möhring J, Böhlen P, Schoun J, Mellet M, Süss U, Schmidt M, Pliska V
Acta Endocrinol (Copenh). 1982 Mar;99(3):371-8. doi: 10.1530/acta.0.0990371.
Radioimmunoassay and chemical assay (high performance liquid chromatography (HPLC) with fluorescence detection) for arginine vasopressin (AVP) in purified synthetic preparations and in posterior lobe tissue samples (ox and rat) yielded essentially identical values. In tissue samples from the neurohypophysis, the reproducibility of the former method was roughly twice as good. The routinely used blood pressure bioassay was very poorly reproducible and yielded values deviating from the two ther methods. In all three assays, the coefficient of variation was roughly five times higher in tissue samples than in synthetic preparations. It is suggested that a chemical rather than a biological assay is used for standardization of AVP for radioimmunoassay. Large differences were found in the quality of AVP preparations potentially usable as standards and tracers (after iodination) for radioimmunoassay. All commercial preparations should therefore be checked for their AVP and impurity content by high performance liquid chromatography.
对纯化的合成制剂以及垂体后叶组织样本（牛和大鼠）中的精氨酸加压素（AVP）进行放射免疫测定和化学测定（带荧光检测的高效液相色谱法（HPLC）），得出的值基本相同。在来自神经垂体的组织样本中，前一种方法的重现性大约是后者的两倍。常规使用的血压生物测定法的重现性非常差，得出的值与其他两种方法不同。在所有三种测定中，组织样本中的变异系数大约是合成制剂中的五倍。建议使用化学测定而非生物测定来对用于放射免疫测定的AVP进行标准化。在有可能用作放射免疫测定标准品和示踪剂（碘化后）的AVP制剂质量方面发现了很大差异。因此，所有商业制剂都应通过高效液相色谱法检查其AVP和杂质含量。
