Babson A L, Opper C A, Crane L J
Am J Clin Pathol. 1982 Apr;77(4):424-9. doi: 10.1093/ajcp/77.4.424.
A rapid, convenient immunologic assay technic for fibrinogen is reported. Basically a latex agglutination, the assay technic, called kinetic latex agglutinometry, quantitates the increase in light transmission that occurs in a stirred suspension of anti-fibrinogen-coated latex beads after the addition of a plasma or whole blood sample containing fibrinogen. In this system the time "delta t" required for a given transmission change to occur is inversely proportional in a log-log relationship to the quantity of fibrinogen in the plasma sample. The reaction is linear over a fibrinogen concentration of greater than 100-fold. The sensitivity can be adjusted over a wide range, and the assay can quantitate as little as 10 ng fibrinogen. The assay can be used with either plasma or whole blood. When compared to the thrombin clotting time method of Clauss, the correlation coefficient is 0.99 for the plasma assay and 0.95 for the whole blood assay. As a one-step assay employing stable reagents and requiring approximately two minutes per assay for normal plasma, the method is ideally suited for use in the clinical laboratory.
本文报道了一种快速、便捷的纤维蛋白原免疫分析技术。该分析技术本质上是一种乳胶凝集法，称为动态乳胶凝集法，通过检测加入含有纤维蛋白原的血浆或全血样本后，包被抗纤维蛋白原乳胶珠的搅拌悬浮液中光透射率的增加来定量纤维蛋白原。在这个系统中，发生给定透射变化所需的时间“Δt”与血浆样本中纤维蛋白原的量呈对数-对数关系的反比。该反应在纤维蛋白原浓度大于100倍的范围内呈线性。灵敏度可在很宽的范围内调节，该分析方法可定量低至10 ng的纤维蛋白原。该分析方法可用于血浆或全血。与Clauss的凝血酶凝血时间法相比，血浆分析的相关系数为0.99，全血分析的相关系数为0.95。作为一种采用稳定试剂的一步分析法，正常血浆每次分析约需两分钟，该方法非常适合临床实验室使用。
