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Salt extractability of nuclear receptor-estrogen complex from the rat uterus.

作者信息

Ikeda M, Omukai Y, Nohno T, Hosokawa K, Senoo T, Saito T

出版信息

Biochim Biophys Acta. 1982 Apr 13;715(2):175-80. doi: 10.1016/0304-4165(82)90356-7.

DOI:10.1016/0304-4165(82)90356-7
PMID:7074136
Abstract

Estrogen receptors in the nuclei of rat uterus were determined 1 or 6 h after the injection of [3H]estradiol. Ratio of 0.4 M KCl-extractable to -resistant receptor-estradiol complex was constant during this time interval. Three consecutive extractions took out about 95% of receptor-estradiol complex either 1 or 6 h after injection. Extraction with KCl before the exchange assay reduced the amount of salt-resistant nuclear receptor-estradiol complex. However, when exchange incubation with [3H] estradiol was done before KCl extraction, salt-resistant receptor-estradiol complex was significantly increased, even after three consecutive extractions.

摘要

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