da Cunha M F, Meistrich M L, Haq M M, Gordon L A, Wyrobek A J
Cancer. 1982 Jun 15;49(12):2459-62. doi: 10.1002/1097-0142(19820615)49:12<2459::aid-cncr2820491207>3.0.co;2-q.
Spermatogenesis in a melanoma patient treated with 12 courses of acridinyl anisidide (AMSA) (20 mg/m2/course) was studied by monitoring sperm concentration, motility, and morphology at various phases of treatment. Chemotherapy was interrupted for 20 weeks between the ninth and tenth course. Sperm concentration and motility began to decline after the second course. At the third course, the percentage of morphologic abnormalities had increased to 86.5% from a pretreatment value of 57.8% (P less than 0.001). Azoospermia was observed at the sixth course and persisted until 12 weeks after the ninth course, when semen levels returned to pretreatment levels: 20 million/ml; 70% motility; 60.1% abnormal forms. Three weeks after the 12th course, the sperm count was reduced to 250,000/ml, motility to 5%, and abnormalities increased to 84.0%. The rapid recovery of normal spermatogenesis observed during the chemotherapy interruption indicates that AMSA has only a temporary, reversible effect on differentiating germinal cells with no toxicity to stem cells.
通过在治疗的不同阶段监测精子浓度、活力和形态，研究了一名接受12个疗程吖啶基茴香胺（AMSA）（20mg/m²/疗程）治疗的黑色素瘤患者的精子发生情况。化疗在第九和第十疗程之间中断了20周。第二疗程后精子浓度和活力开始下降。在第三疗程时，形态异常的百分比从治疗前的57.8%增加到了86.5%（P<0.001）。在第六疗程时观察到无精子症，这种情况一直持续到第九疗程后12周，此时精液水平恢复到治疗前水平：2000万/ml；活力70%；异常形态60.1%。第12疗程后3周，精子计数降至25万/ml，活力降至5%，异常率增加到84.0%。化疗中断期间观察到的正常精子发生的快速恢复表明，AMSA对分化中的生殖细胞只有暂时的、可逆的影响，对干细胞无毒性。
