Esteves W, Soares L A, Hartman L, Pereira A S
Clin Chem. 1982 Apr;28(4 Pt 1):603-5.
In this method for determining fecal lipids, feces are saponified with ethanolic potassium hydroxide, the ethanol is evaporated, and the acidified residue extracted with hexane. An aliquot of the hexane solution is then titrated with ethanolic sodium hydroxide, the solvents are evaporated, and the residue (soaps and unsaponifiable matter) is weighed. The unsaponifiable matter is extracted with acetone and determined gravimetrically. The method thus permits the determination of milli-equivalents of fatty acids, total lipids, and fatty acids in one sample and is applicable to feces containing medium-chain fatty acids and their triglycerides.
在这种测定粪便脂质的方法中，粪便用乙醇氢氧化钾皂化，乙醇蒸发掉，酸化后的残渣用己烷萃取。然后取一份己烷溶液用乙醇氢氧化钠滴定，溶剂蒸发掉，残渣（肥皂和不皂化物）称重。不皂化物用丙酮萃取并用重量法测定。该方法能够在一个样品中测定脂肪酸的毫当量、总脂质和脂肪酸，适用于含有中链脂肪酸及其甘油三酯的粪便。
