Kim J B, Barnard G J, Collins W P, Kohen F, Lindner H R, Eshhar Z
Clin Chem. 1982 May;28(5):1120-4.
We describe a simple, solid-phase chemiluminescence immunoassay for the measurement of estradiol-17 beta in extracts of peripheral venous plasma. The method has similar sensitivity, specificity, precision, and accuracy to a conventional radioimmunoassay with a tritiated antigen. An immunoglobulin G fraction od monoclonal antibodies to estradiol-6-carboyxmethyl oxime-bovine serum albumin is passively adsorbed onto the walls of polypropylene tubes. The labeled antigen is estradiol-6-carboxymethyl oxime-aminobutylethyl isoluminol. After the binding reaction (1 h at 22 degrees C), the solution is removed by aspiration (400 microliters). Sodium hydroxide (5 mol/L, 300 microliters) is added and the mixture incubated for 30 min at 37 degrees C. Luminescence is initiated by oxidation of the label with microperoxidase/hydrogen peroxide and the signal integrated for 10 s. The light yield is inversely proportional to the concentration of estradiol in the standard or sample.
我们描述了一种用于测量外周静脉血浆提取物中雌二醇-17β的简单固相化学发光免疫分析方法。该方法与使用氚标记抗原的传统放射免疫分析具有相似的灵敏度、特异性、精密度和准确度。将抗雌二醇-6-羧甲基肟-牛血清白蛋白的单克隆抗体的免疫球蛋白G组分被动吸附到聚丙烯管的管壁上。标记抗原为雌二醇-6-羧甲基肟-氨基丁基乙基异鲁米诺。结合反应（22℃下1小时）后，通过抽吸去除溶液（400微升）。加入氢氧化钠（5mol/L，300微升），混合物在37℃下孵育30分钟。通过用微过氧化物酶/过氧化氢氧化标记物引发发光，并将信号积分10秒。光产率与标准品或样品中雌二醇的浓度成反比。
