Christenson R H, McGlothlin C D, Hedrick R, Cate J C
Clin Chem. 1982 May;28(5):1204-7.
We describe a simplified fluorometric method for quantitatively determining metanephrines in urine. The method is based on the elution of metanephrines from an ion-exchange column followed by production of a fluorescent derivative that has excitation and emission maxima of 405 nm and 505 nm, respectively. Metanephrine concentrations as great as 7.0 mg/L were within the linear range of the assay. Analytical recovery of metanephrines was 82-87%. Cross contamination by catecholamines was insignificant. The upper range of normal was 520 micrograms/24 h. Within-run and between-run CV's were 5% and about 9%, respectively. Twenty-four-hour urine specimens from five patients with pheochromocytoma showed above-normal amounts of the metanephrines by this method.
我们描述了一种用于定量测定尿中变肾上腺素的简化荧光法。该方法基于变肾上腺素从离子交换柱上洗脱，随后生成一种荧光衍生物，其激发和发射最大值分别为405nm和505nm。变肾上腺素浓度高达7.0mg/L在该测定的线性范围内。变肾上腺素的分析回收率为82 - 87%。儿茶酚胺的交叉污染不显著。正常上限为520微克/24小时。批内和批间变异系数分别为5%和约9%。通过该方法，来自五名嗜铬细胞瘤患者的24小时尿液标本显示变肾上腺素含量高于正常。
