Zippel R, Martegani E, Vanoni M, Mazzini G, Alberghina L
Cytometry. 1982 May;2(6):426-30. doi: 10.1002/cyto.990020612.
A new procedure to determine the durations of cell cycle phases is based on th autoradiography of labeled nuclei and a standard chemical determination of DNA content per cell. It has been used to study the cell cycle timing of a human established cell line, EUE cells, so far poorly characterized in its kinetic aspects. The cycle phase durations obtained with this method are compared with the DNA frequency profiles determined by flow cytometry and found to agree closely. The relevance of the growth conditions of the exponentially growing cultures for a correct estimation of the cycle phase duration is indicated. Cell size distribution has been shown to vary according to growth condition.
一种确定细胞周期各阶段时长的新方法，是基于标记细胞核的放射自显影术以及对每个细胞DNA含量的标准化学测定。该方法已用于研究人类已建立的细胞系EUE细胞的细胞周期时间，到目前为止，其动力学方面的特征尚不明确。将用此方法获得的细胞周期阶段时长，与通过流式细胞术测定的DNA频率分布图进行比较，发现二者吻合度很高。结果表明，指数生长培养物的生长条件对于正确估计细胞周期阶段时长具有重要意义。细胞大小分布已被证明会因生长条件而异。
