冷冻保存过程对人类精子摄取铷的影响。

The influence of the cryopreserving process on rubidium uptake by human spermatozoa.

作者信息

Glander H J, Haustein U F

出版信息

Int J Biol Res Pregnancy. 1982;3(2):60-5.

Abstract

The effect of cryopreservation and its phases on the netto-Rb+ uptake (analogous to the K+ uptake) of washed human spermatozoa was investigated under different conditions. The Rb+ uptake reached a steady state after almost 50-min incubation both before and after the cryopreserving process. The Rb+ uptake could be reduced to about 30% by ouabain and to about 80% by monoiodoacetate. The cryoprotective agents glycerol (G), ethylene glycol (EG), and particularly dimethylsulfoxide (DMSO) reduced the Rb+ uptake by a factor dependent on its concentration. The comparative cryopreservation with G, EG, DMSO,and dimethylformamide (DMFA) as cryoprotective agents caused an inhibition especially of the ouabain-sensitive netto-Rb+ uptake. This inhibitory effect became stronger from G to EG and from DMSO to DMFA. Within the course of cryopreservation especially the process of pelletation, Rb+ uptake was reduced, whereas the prolongation of the equilibration time up to 180 min moderated the effect of the cryopreservation on the Rb+ uptake. Beside the reduction of the absolute Rb+ uptake, the cryopreservation altered the dependence of the netto-Rb+ uptake on the extracellular Rb+ concentration in a sigmoid manner.

摘要

在不同条件下，研究了冷冻保存及其各阶段对洗涤后的人类精子净Rb⁺摄取（类似于K⁺摄取）的影响。在冷冻保存过程前后，经过近50分钟的孵育后，Rb⁺摄取均达到稳定状态。哇巴因可将Rb⁺摄取降低至约30%，碘乙酸可将其降低至约80%。冷冻保护剂甘油（G）、乙二醇（EG），尤其是二甲基亚砜（DMSO），会根据其浓度降低Rb⁺摄取。以G、EG、DMSO和二甲基甲酰胺（DMFA）作为冷冻保护剂进行的比较冷冻保存尤其会抑制对哇巴因敏感的净Rb⁺摄取。这种抑制作用从G到EG以及从DMSO到DMFA变得更强。在冷冻保存过程中，尤其是沉淀过程中，Rb⁺摄取减少，而将平衡时间延长至180分钟可减轻冷冻保存对Rb⁺摄取的影响。除了绝对Rb⁺摄取量减少外，冷冻保存还以S形方式改变了净Rb⁺摄取对细胞外Rb⁺浓度的依赖性。