Shin M, Sakihama N, Sugimoto N, Miyazawa T, Oshino R
J Biochem. 1982 Mar;91(3):953-7. doi: 10.1093/oxfordjournals.jbchem.a133785.
Immobilized ferredoxin-NADP+ reductase (FNR) was prepared by coupling reaction of CNBr-Sepharose 4B with the spinach enzyme. The immobilized FNR was found to retain the activity of complex formation with ferredoxin as well as the enzymatic activities such as NADPH-diaphorase and NADPH-cytochrome c reductase activities. The complex formation of immobilized FNR with ferredoxin was investigated by measuring reflex spectra of the immobilized FNR with or without ferredoxin and by titration with ferredoxin. The experimental results obtained for the dissociation constant, pH profile and effect of salts were coincidental with those reported for the free enzyme system.
通过将溴化氰活化的琼脂糖4B与菠菜中的铁氧化还原蛋白-NADP⁺还原酶（FNR）进行偶联反应制备了固定化的FNR。发现固定化的FNR保留了与铁氧化还原蛋白形成复合物的活性以及诸如NADPH-黄递酶和NADPH-细胞色素c还原酶活性等酶活性。通过测量有或没有铁氧化还原蛋白时固定化FNR的反射光谱以及用铁氧化还原蛋白进行滴定，研究了固定化FNR与铁氧化还原蛋白的复合物形成。解离常数、pH曲线和盐效应的实验结果与游离酶系统报道的结果一致。
