器官培养中兔附睾精子的肉碱含量

Carnitine content of rabbit epididymal spermatozoa in organ culture.

作者信息

Casillas E R, Chaipayungpan S

出版信息

J Reprod Fertil. 1982 May;65(1):247-51. doi: 10.1530/jrf.0.0650247.

DOI:10.1530/jrf.0.0650247

Abstract

Tubules of the rabbit proximal corpus epididymis were maintained for 24 h in vitro. The carnitine content in cells from tubules cultured with dihydrotestosterone was greater than that in cells from tubules cultured without androgen. The effect of dihydrotestosterone was abolished by the simultaneous addition of cyproterone acetate to the medium. Culture of segments of the distal corpus for 24 h with dihydrotestosterone did not change the carnitine content of spermatozoa. These results support our hypothesis that the accumulation of carnitine is involved in sperm maturation.

摘要

将兔附睾近侧体的小管进行体外培养24小时。用双氢睾酮培养的小管细胞中的肉碱含量高于未用雄激素培养的小管细胞中的肉碱含量。向培养基中同时添加醋酸环丙孕酮可消除双氢睾酮的作用。用双氢睾酮对远侧体节段进行24小时培养，并未改变精子的肉碱含量。这些结果支持了我们的假设，即肉碱的积累参与精子成熟过程。