使用放射性标记抗原定量被动皮肤过敏反应（PCA）。
Quantitation of passive cutaneous anaphylaxis (PCA) by using radiolabelled antigen.
作者信息
Ring J, Seifert J, Brendel W
出版信息
Allergy. 1978 Jun;33(3):138-42. doi: 10.1111/j.1398-9995.1978.tb01523.x.
The major problem of detecting reaginic antibody by passive cutaneous anaphylaxis (PCA) is the quantitation of the dye reaction. Radiolabelled antigen was used in an attempt to quantitate the PCA reaction (Radio-PCA). Antisera containing reaginic antibody against human serum albumin (HSA) were produced in rabbits. These antisera were injected into normal rabbit skin in different dilutions. Twenty-four hours later HSA was injected intravenously either with Evans Blue or as 125-I-HSA. Radioactivity found in antibody-containing skin was significantly higher than in control specimens containing saline or normal rabbit serum, as low as antiserum dilutions of 1:1,000. Compared with the Evans Blue technique Radio-PCA was able to distinguish quantitatively between different antiserum dilutions at a higher level of statistical significance.
通过被动皮肤过敏反应（PCA）检测反应素抗体的主要问题是染料反应的定量。为了对PCA反应进行定量，使用了放射性标记抗原（放射PCA）。在兔体内制备了含抗人血清白蛋白（HSA）反应素抗体的抗血清。将这些抗血清以不同稀释度注射到正常兔皮肤中。24小时后，静脉注射HSA，同时注射伊文思蓝或作为¹²⁵I-HSA。在含抗体的皮肤中发现的放射性明显高于含生理盐水或正常兔血清的对照标本，抗血清稀释度低至1:1000。与伊文思蓝技术相比，放射PCA能够在更高的统计学显著性水平上对不同抗血清稀释度进行定量区分。
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