Ambre J J, Ruo T I, Smith G L, Backes D, Smith C M
J Anal Toxicol. 1982 Jan-Feb;6(1):26-9. doi: 10.1093/jat/6.1.26.
Most assays for the identification of cocaine use are aimed at detection of benzoylecgonine, a major urinary metabolite. Gas chromatography assays require derivatization of benzoylecgonine. Recently, ecgonine methyl ester (methylecgonine) has been shown to be major urinary metabolite of cocaine in man after oral administration. These results indicate that ecgonine methyl ester is a prominent urinary metabolite after street use of cocaine, which primarily involves intranasal application and intravenous injection rather than ingestion. Advantages of GC/MS analysis for ecgonine methyl ester in identifying cocaine use include the ability to chromatograph it on common stationary phases without derivatization and its early elution.
大多数用于鉴定可卡因使用情况的检测方法旨在检测主要的尿液代谢物苯甲酰爱康宁。气相色谱检测需要对苯甲酰爱康宁进行衍生化。最近，芽子碱甲酯（甲基芽子碱）已被证明是人体口服可卡因后的主要尿液代谢物。这些结果表明，在街头使用可卡因（主要涉及鼻内给药和静脉注射而非摄入）后，芽子碱甲酯是一种显著的尿液代谢物。气相色谱/质谱联用（GC/MS）分析芽子碱甲酯在鉴定可卡因使用情况方面的优势包括能够在不进行衍生化的情况下在常见固定相上对其进行色谱分析以及其较早洗脱。
