Newman S M, Summitt R L, Nunez L J
Mutat Res. 1982 Mar;101(1):67-75. doi: 10.1016/0165-1218(82)90166-5.
Nickel is a recognized carcinogen to which the general population is being increasingly exposed. A nickel-induced alteration in the incidence of sister-chromatid exchanges in cultured lymphocytes has been investigated. Human lymphocytes from a single donor were cultured in media containing various concentrations of nickelous chloride from 10(-6) to 10(-3) M and a control culture containing no added nickel. A significantly increased incidence of exchanges over background was induced at 1.19 x 10(-4) M as indicated by a Student's t-test. A concentration/response curve was generated up to 5 x 10(-4) M where the nickel concentration was lethal to the cells. The nickel response was analyzed by a chi-square test for any variation in location of activity across the karyotype and no differences were detected. The background incidence did vary from a uniform distribution across the karyotype with increasing incidences in chromosomes 1 and 2 and those of the B group, and decreased incidences in the E, F, and G groups.
镍是一种公认的致癌物，普通人群接触到它的情况越来越多。人们对镍诱导培养淋巴细胞中姐妹染色单体交换发生率的改变进行了研究。从一名供体获取的人类淋巴细胞在含有浓度从10(-6)到10(-3)M的各种氯化镍的培养基中培养，以及一个未添加镍的对照培养物。经学生t检验表明，在1.19×10(-4)M时诱导的交换发生率显著高于背景值。生成了一条直至5×10(-4)M的浓度/反应曲线，此时镍浓度对细胞具有致死性。通过卡方检验分析镍反应，以检测整个核型中活性位置的任何变化，未检测到差异。背景发生率确实不同于整个核型的均匀分布，1号和2号染色体以及B组染色体的发生率增加，而E、F和G组的发生率降低。
