Wójcik M, Ułas J, Oderfeld-Nowak B
Neuroscience. 1982 Feb;7(2):495-9. doi: 10.1016/0306-4522(82)90282-2.
The effect of intramuscular administration of a mixture of gangliosides (21% GM1, 39.7% GD1a, 16% GD1b, 19% GT1) in a daily of 50 mg per kg upon the time course of changes in hippocampal acetylcholinesterase and choline acetyltransferase activities after extensive medioventral septal lesions in the rat was checked on days 3, 5, 18 and 50 after the operation. Following the early decrease in the enzyme activities to about 25% of control due to degeneration, a gradual recovery up to about 50% of control activity at the 50th day was found. When gangliosides were administered, the recovery in the activity of both enzyme was more pronounced. The ratio of the enzyme activities from the animals injected with gangliosides to that from uninjected animals was 1.45 and 1.48 on the 18th day and 1.62 and 1.50 on the 50th day after the operation, for choline acetyltransferase and acetylcholinesterase activity, respectively. Since no significant effect of ganglioside injection was seen at early post-operative times i.e. on days 3 and 5, the effects seen on days 18 and 50 seem to be specifically due to facilitation of the recovery processes and not to retardation of the degeneration processes. Assuming that the spontaneous recovery of cholinergic enzyme activity reflects reinnervation of the hippocampus through collateral sprouting gangliosides would seen to facilitate the regrowth of new cholinergic nerve terminals.
在大鼠进行广泛的中腹隔损伤后，于术后第3、5、18和50天检查了每日每千克50毫克的神经节苷脂混合物（21% GM1、39.7% GD1a、16% GD1b、19% GT1）肌肉注射对海马乙酰胆碱酯酶和胆碱乙酰转移酶活性变化时间进程的影响。由于变性，酶活性早期下降至对照的约25%，之后发现逐渐恢复，在第50天时达到对照活性的约50%。当给予神经节苷脂时，两种酶活性的恢复更为明显。术后第18天，注射神经节苷脂的动物与未注射动物的酶活性之比，胆碱乙酰转移酶活性为1.45，乙酰胆碱酯酶活性为1.48；术后第50天，该比值分别为1.62和1.50。由于在术后早期即第3天和第5天未观察到神经节苷脂注射的显著影响，因此在第18天和第50天观察到的影响似乎具体是由于促进了恢复过程，而不是延缓了变性过程。假设胆碱能酶活性的自发恢复反映了通过侧支发芽对海马的再支配，那么神经节苷脂似乎有助于新的胆碱能神经末梢的再生。
