无需灰化的激素碘半自动测定法。
Semi-automated determination of hormonal iodine without incineration.
作者信息
Cabello G, Michel M C
出版信息
Ann Rech Vet. 1978;9(1):89-98.
A sensitive semi automated technique for the determination of hormonal iodine is described. The thyroid hormones are isolated by means of a cation exchange resin (Dowex 50 Wx2). The iodine atoms are liberated from the organic molecules with bromine, without prior incineration or destruction (moidified Pileggi's method). Then, mineral iodine is measured colorimetrically with a Technicon autoanalyzer. This technique seems particularly adapted to a programme of standardisation because of its sensitivity and reproductibility. It is well adapted to the determination of circulating hormonal iodine of the ruminants, in which the concentration is lower than in man.
本文描述了一种用于测定激素碘的灵敏半自动技术。甲状腺激素通过阳离子交换树脂（Dowex 50 Wx2）进行分离。碘原子无需预先灰化或破坏（改良的皮莱吉法），而是用溴从有机分子中释放出来。然后，用Technicon自动分析仪比色测定无机碘。由于该技术的灵敏度和可重复性，它似乎特别适用于标准化方案。它非常适合测定反刍动物循环激素碘，反刍动物体内该激素的浓度低于人类。
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