DiMichele L, Powers D A
Science. 1982 May 28;216(4549):1014-6. doi: 10.1126/science.7079747.
Adenosine triphosphate levels in erythrocytes are correlated with LDH-B genotype in Fundulus heteroclitus. Adenosine triphosphate is the fish's allosteric modifier of hemoglobin oxygen affinity. Since oxygen delivery to muscle affects swimming performance, fish of each homozygous LDH-B phenotype were swum to exhaustion at 10 degrees or 25 degrees C to determine whether in vitro differences attributed to the LDH-B allelic isozymes were manifest in vivo. At 10 degrees C, the critical swimming speed of the LDH-BaBa phenotype was 3.6 body lengths per second, whereas that of the LDH-BbBb phenotype was 4.3 body lengths per second. At 25 degrees C there were no differences between LDH-B phenotypes in erythrocyte adenosine triphosphate levels, blood oxygen affinity, or swimming performance.
在底鳉中，红细胞中的三磷酸腺苷水平与乳酸脱氢酶-B（LDH-B）基因型相关。三磷酸腺苷是该鱼类血红蛋白氧亲和力的别构调节剂。由于氧气输送到肌肉会影响游泳表现，因此将每种纯合LDH-B表型的鱼在10摄氏度或25摄氏度下游泳至精疲力竭，以确定体外归因于LDH-B等位基因同工酶的差异在体内是否明显。在10摄氏度时，LDH-BaBa表型的临界游泳速度为每秒3.6个体长，而LDH-BbBb表型的临界游泳速度为每秒4.3个体长。在25摄氏度时，LDH-B表型在红细胞三磷酸腺苷水平、血氧亲和力或游泳表现方面没有差异。
